Figure 1. C/EBPβ confocal fluorescent immunocytochemistry in human microglia cultures.

Parallel wells received (A) no cytokine treatment or 50 ng/ml (B) IL-1β, (C) IL-6, or (D) TNF-α for 4 hours. Microglia treated with cytokines show increased expression and nuclear localization of C/EBPβ. All wells were imaged concurrently with the same photomultiplier tube intensity, gain, and offset settings using an Olympus Fluoview Confocal microscope–200X (scale bar = 100 µm).