In total 45 CYP5035 P450 sequences from five model basidiomycetes (Fig. 1) were included in the tree. The minimum evolution tree was constructed using the close-neighbor-interchange algorithm in MEGA (version 5.05). For ease of visual identity, the tree branch color, protein name, protein ID (parenthesis) and model basidiomycete species name were presented with unique color. The protein size in amino acids is also shown in the figure. The gene-structure analysis for each P450 was presented in the form of exon-intron organization. A graphical format showing parallel (gene size) and vertical lines (introns) is presented for P450s showing similar gene structure. For the rest of the P450s, the number of exons and introns was shown. The genetic location of P450 is shown in the form of the scaffold number. Abbreviations: Pchr, Phanerochaete chrysosporium; Pcar, Phanerochaete carnosa; Abis, Agaricus bisporus; Gsp, Ganoderma sp.; Ppla, Postia placenta; Slac, Serpula lacrymans.