Figure 6. Phylogenetic and gene-structure analysis of CYP5136 family.

Twenty-four P450 sequences from four model basidiomycetes Phanerochaete chrysosporium (Pchr), Phanerochaete carnosa (Pcar), Ganoderma sp. (Gsp) and Serpula lacrymans (Slac) were included in the tree. A minimum evolution tree was constructed using the close-neighbor-interchange algorithm in MEGA (version 5.05). For ease of visual identity, the tree branch color, protein name, protein ID (parenthesis) and model basidiomycete species name were presented in red (Pchr) and pink (Pcar). The protein size in amino acids is also shown in the figure. Gene-structure analysis for each P450 was presented in the form of exon-intron organization. A graphical format showing parallel (gene size) and vertical lines (introns) is presented for P450s showing similar gene structure (highlighted with unique background color). For the rest of the P450s, the number of exons and introns was shown. The genetic location of P450 is shown in the form of the scaffold number.