Figure 9. Phylogenetic and gene-structure analysis of CYP5146 family.

Eighteen P450 sequences from two white rot basidiomycetes, Phanerochaete chrysosporium (Pchr) and Phanerochaete carnosa (Pcar), were included in the tree. The minimum evolution tree was constructed using the close-neighbor-interchange algorithm in MEGA (version 5.05). For ease of visual identity, the tree branch color, protein name, protein ID (parenthesis) and model basidiomycete species name were presented in red (Pchr) and pink (Pcar). The protein size in amino acids is shown in the figure. The gene-structure analysis for each P450 was presented in the form of exon-intron organization. A graphical format showing parallel (gene size) and vertical lines (introns) is presented for P450s showing similar gene structure (highlighted with unique color). For the rest of the P450s, the number of exons and introns was shown. The genetic location of P450 is shown in the form of the scaffold number.