|
P. aeruginosa
|
|
|
| PAO1 |
Wild-type strain |
ATCC15692 |
| PAO1/pBBR |
PAO1 harbouring pBBR1MCS-4 |
This study |
| PAO1::Tn7T |
PAO1 transposed with pUC18-mini-Tn7T-Gm-LAC |
This study |
| ΔiscR
|
PAO1 ΔiscR mutant |
This study |
| ΔiscR/pBBR |
ΔiscR mutant harbouring pBBR1MCS-4 |
This study |
| ΔiscR::Tn7T |
ΔiscR mutant transposed with pUC18-mini-Tn7T-Gm-LAC |
This study |
| ΔiscR::iscR
|
ΔiscR mutant transposed with pTn7TiscR
|
This study |
| ΔiscR::iscSUA-hscBA-fdx2-iscX
|
ΔiscR mutant transposed with pTn7TiscSUA-hscBA-fdx2-iscX
|
This study |
|
E. coli
|
|
|
| DH5α |
φ80d lacZΔM15, recA1, endA1, gyrA96, thi-1, hsdR17(rk
−, mk
+), supE44, relA1, deoR, Δ(lacYMA-argF)U169 |
Stratagene Inc.(USA) |
|
Plasmid
|
|
|
| pUC18-mini-Tn7T-Gm-LAC |
pUC18 containing mini-Tn7T::Plac site, Apr, Gmr
|
[29]
|
| pTn7TiscR
|
pUC18-mini-Tn7T-Gm-LAC containing iscR
|
This study |
| pBBR1MCS-4 |
Medium-copy-number expression vector, Apr
|
[32]
|
| pBBRiscR
|
pBBR1MCS-4 containing iscR
|
This study |
| pBBRiscR-C92A |
pBBRiscR with C92A mutagenesis |
This study |
| pBBRiscR-C98A |
pBBRiscR with C98A mutagenesis |
This study |
| pBBRiscR-C104A |
pBBRiscR with C104A mutagenesis |
This study |
| pBBRiscR-H107A |
pBBRiscR with H107A mutagenesis |
This study |
| pBBRiscR-E43A |
pBBRiscR with E43A mutagenesis |
This study |
| pBBRiscR-C111A |
pBBRiscR with C111A mutagenesis |
This study |
| pCM351 |
vector containing the loxP-Gmr-loxP region, Gmr
|
[30]
|
