| BT2781 |
GCCCGCACAAGCGGTGGAG
|
Forward primer for 16S ribosomal gene |
| BT2782 |
ACGTCATCCCCACCTTCCT
|
Reverse primer for 16S ribosomal gene |
| BT3210 |
CGAGGTAGATCGGCAATT
|
Reverse primer for full-length iscR
|
| BT3577 |
CCTGCTGTCGGGTAACGC
|
Reverse primer for primer extension |
| BT3578 |
ATCATGCGCGAGGACTCC
|
Forward primer for iscR deletion |
| BT3579 |
GGATCGGCGTTGACCAGC
|
Reverse primer for iscR deletion |
| BT3555 |
CGCAATGGCATCGAGATCGA
|
Forward primer for fdx2 expression |
| BT3556 |
GATAGCCGCGAATCGGGCTC
|
Reverse primer for fdx2 expression |
| BT3584 |
CACCTGTGGGCCGACCTCAGT
|
Site-directed mutagenesis of IscR-C111A |
| BT3585 |
CTGAGGTCGGCCCACAGGTGG
|
Site-directed mutagenesis of IscR-C111A |
| BT3612 |
GAAGATTTCGCCGGAGTCAA
|
Forward primer for iscR promoter fragment |
| BT3613 |
GCGTTCGGAGATATCGGCCAG
|
Reverse primer for iscR expressionand iscR promoter fragment |
| EBI102 |
GCGACCCGCGCCCAGGGGCAG
|
Site-directed mutagenesis of IscR-C92A |
| EBI103 |
CTGCCCCTGGGCGCGGGTCGC
|
Site-directed mutagenesis of IscR-C92A |
| EBI120 |
ACCCCGAATGATCCCGATG
|
Forward primer for iscR expression |
| EBI121 |
GGAAAAGCCCATGCGTCTGA
|
Forward primer for full-length iscR
|
| EBI142 |
CAGGGCGATGCCCACTCCGGC
|
Site-directed mutagenesis of IscR-C98A |
| EBI143 |
GCCGGAGTGGGCATCGCCCTG
|
Site-directed mutagenesis of IscR-C98A |
| EBI144 |
GGCGATACCGCTCTGACCCAC
|
Site-directed mutagenesis of IscR-C104A |
| EBI145 |
GTGGGTCAGAGCGGTATCGCC
|
Site-directed mutagenesis of IscR-C104A |
| EBI148 |
TCCTATCTCGCACAGCTGTTC
|
Site-directed mutagenesis of IscR-E43A |
| EBI149 |
GAACAGCTGTGCGAGATAGGA
|
Site-directed mutagenesis of IscR-E43A |
| EBI192 |
TGTCTGACCGCCCACCTGTGG
|
Site-directed mutagenesis of IscR-H107A |
| EBI193 |
CCACAGGTGGGCGGTCAGACA
|
Site-directed mutagenesis of IscR-H107A |
| M13F |
GTAAAACGACGGCCAGT
|
Universal forward primer for expression vector |
| M13R |
AAACAGCTATGACCATG
|
Universal reverse primer for expression vector |
