ZmMADS2 promoter deletion analyses with transiently transformed pollen and leaves of maize and tobacco. a, Relative expression levels of luciferase directed by ZmMADS2 promoter deletion constructs in pollen and leaves of maize and tobacco, respectively. Ten experiments were conducted with pDNS-5, -6, -6b, -7, and -8, whereas three experiments were conducted with pDNS-9 and -10. The promoterless control was used in more than 30 experiments. Vertical lines within bars indicate relative minimum and maximum rates, and bars indicate average expression levels. For a better comparison, expression levels of pDNS-6b were set to 100% for each individual experiment. b, Deletion constructs consist of boxes and putative cis elements as shown in Figure 2c. Relative luciferase expression in pollen of maize and tobacco as an average of 10 experiments is shown. n.d., Not determined.