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. 2014 Jan 22;9(1):e86897. doi: 10.1371/journal.pone.0086897

Figure 9. Distribution of cells according to its ParB localization.

Figure 9

DIC, mCherry-ParB (in green), DAPI (in blue), and ParA-YFP (in red) microscope fluorescence images of cells from the strain MR2526 (Pvan-parA-yfp, PIPTG -mCherry-parB) grown without vanillate, and with IPTG (1 mM) during 3 hours for mCherry-parB expression. Black scale bar represents 5 µm. A total of 550 cells from three independent experiments were examined and the mean and standard deviation are reported. (A) Cell having one chromosomal mass and a single mCherry-ParB focus at the edge of the nucleoid (12.7±1.8%). (B) Cell having one chromosomal mass, a mCherry-ParB focus at the edge of the nucleoid and another mCherry-ParB focus in an intermediate nucleoid position (11.3±0.3%). (C) Cell presenting one chromosomal mass, and two mCherry-ParB foci at both edges of a single nucleoid (50.7±3.7%). (D) Cell having two chromosomal masses, and two mCherry-ParB foci at the subpolar edges of both nucleoids (22.5±1.7%). (E) The same as in (D) but with some sign of cellular pinch at the cell division plane (2.8±1.5%).