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. 2014 Jan 22;9(1):e86842. doi: 10.1371/journal.pone.0086842

Figure 2. Identification of an extracellular Hsp90 complex containing the ECM protein FN.

Figure 2

(A) Surface proteins of MDA-MB-231 cells were chemically crosslinked with the thiol-cleavable DTSSP reagent and cell lysates immunoprecipitated with Hsp90α/β primary antibody. Crosslinked samples were resolved by non-reducing SDS-PAGE followed by colloidal Coomassie staining. Four bands (labeled A–D) were excised from the gel and analysed by tandem mass spectrometry (MS/MS). The dotted line represents the stacking and resolving gel interface. (B) Data summary from the MS/MS analysis indicated the presence of Hsp90α/β (Band/sample A–D) and human FN1 (Band/sample A). The mass refers to the molecular weight of the respective proteins (in Daltons). A higher protein score indicates a more confident match. The queries matched refer to the number of sequences with a direct match to the query protein. The exponentially modified protein abundance index (emPAI) refers to the relative abundance of the query protein in each sample.