Figure 1.

Time-dependent effect of heat shock on cell viability. One hundred milliliters of TBY-2 cell cultures was treated for 10 min at 55°C and returned to 27°C for a 72-h period. At indicated time intervals after heat treatment, viability of both control (○) and heat-shocked (•) cells was measured using trypan blue staining and examination by phase contrast microscopy. In both cases, the percentage (±se) of viable cells was counted in a population of at least 1,000 cells in six separate experiments.