Figure 4.

Production of H₂O₂ in PCD-induced cells. TBY-2 cells were kept either at 27°C or taken immediately after heat shock at 55°C. The cells were stained with DHR123, and H₂O₂ production was visualized by fluorescent microscopy as described in “Materials and Methods.” Cells were photographed under both fluorescent field and phase contrast. Pictures represent typical examples. Bar = 20 μm.