Figure 5.

Release of H₂O₂ into the culture medium by TBY-2 cells. The concentration of H₂O₂ was measured in the absence (black columns) or in the presence of 0.4 mm ASC (gray columns) added to the culture medium before heat shock. The concentration of H₂O₂ in control cells (white columns) was also measured. At the indicated times, 1 mL of cell suspension (0.12 g of cells) was taken, and the concentration of H₂O₂ was determined in the medium as described in “Materials and Methods.” Values represent mean (±se) of five experiments. An asterisk indicates the values that are significantly different from the control (P < 0.01).