Figure 9.

Kinetic behavior of cAPX in control and PCD cells. The analysis was performed in extracts from control cells (○) and from cells 6 h after the heat shock (•). A, Concentration of H₂O₂ was 170 μm, and the concentration of ASC was varied over the range 10 to 700 μm. B, Concentration of ASC was 500 μm, and the concentration of H₂O₂ was varied over the range 5.5 to 330 μm. C, V_max and K_m values for ASC and H₂O₂ in control and heat-shocked cells. Values represent the mean (±se) of five experiments.