Table II.

Metabolism of ent-kaurene by yeast expressing PsKO cDNA clones

Yeast Strain	ent-Kaurene	ent-Kaurenol	ent-Kaurenal	ent-Kaurenoic Acida
WAT11 PsKO	√	×	×	√
WAT11 lh-1	√	×	×	×
WAT11	√	×	×	×
WAT21 PsKO	√	×	×	√
WAT21 lh-1	√	×	×	×
WAT21	√	×	×	×

^aent-kaurenoic acid methyl ester product was identified by gas chromatography (GC)-mass spectrometry (MS) and compared with an authentic standard. Kovats retention index for both was 2,342. Comparison of spectra: ion peak heights (percentage relative abundance for WAT21 PsKO product/ent-kaurenoic acid standard): 316 [M⁺] (29/30), 301 (22/23), 273 (44/43), 257 (87/98), 256 (46/44), 241 (100/100), 213 (45/61), 199 (23/26), 187 (32/27), and 159 (36/41)