Table II.
Yeast Strain | ent-Kaurene | ent-Kaurenol | ent-Kaurenal | ent-Kaurenoic Acida |
---|---|---|---|---|
WAT11 PsKO | √ | × | × | √ |
WAT11 lh-1 | √ | × | × | × |
WAT11 | √ | × | × | × |
WAT21 PsKO | √ | × | × | √ |
WAT21 lh-1 | √ | × | × | × |
WAT21 | √ | × | × | × |
ent-kaurenoic acid methyl ester product was identified by gas chromatography (GC)-mass spectrometry (MS) and compared with an authentic standard. Kovats retention index for both was 2,342. Comparison of spectra: ion peak heights (percentage relative abundance for WAT21 PsKO product/ent-kaurenoic acid standard): 316 [M+] (29/30), 301 (22/23), 273 (44/43), 257 (87/98), 256 (46/44), 241 (100/100), 213 (45/61), 199 (23/26), 187 (32/27), and 159 (36/41)