Skip to main content
. 2004 Mar;134(3):1181–1190. doi: 10.1104/pp.103.035634

Figure 2.

Figure 2.

Northern-blot analysis of TPI mRNA and TPI activity (mean ± sem) in leaves growing at node +1. RNA gel-blot analysis of pi gene transcripts of unelicited control (CON) and hornworm-elicited (CAT) plants 24 h after the start of feeding (top band, TPI mRNA: 1.4 kb, bottom band, 18S rRNA: 3.4 kb). TPI activity (mean ± sem) in CON- and CAT-elicited plants 3 d after elicitation (n.d., Not detectable in A genotype). A, Untransformed WT N. attenuata plants of the Utah genotype (WT), two homozygous T3 independently transformed lines of the Utah genotype that had been transformed with a construct containing a 175-bp pi gene fragment in an antisense orientation (AS–and AS-), or with an empty vector construct (C). B, Untransformed plants of the A genotype, and plants of the Arizona genotype transformed with a construct containing the full-length pi gene in a sense orientation (S+ and S++), or with an empty vector construct (AC). Hornworm caterpillars fed on leaves growing at node +1 (one position older than the source-sink transition leaf: node 0) at the rosette stage.