Table 1.
Numbers of significantly regulated genes, categorised according to which factors and/or interactions were significant in the three-way ANOVA
| Category | Significant genes | Biological interpretation |
|---|---|---|
|
A Maternal nutrition |
368 (151) |
Maternal nutritional status has an effect independent of leptin treatment and postweaning diet |
|
B Leptin treatment |
200 (90) |
Leptin treatment has an effect independent of maternal nutritional status and postweaning diet |
|
C Postweaning diet |
990 (531) |
Postweaning diet has an effect independent of maternal nutritional status and leptin treatment |
|
AB (2-way interaction) |
949 (503) |
The effect of leptin treatment is dependent on maternal nutritional status (or vice versa) |
|
AC (2-way interaction) |
96 (18) |
The effect of postweaning diet is dependent on maternal nutritional status (or vice versa) |
|
BC (2-way interaction) |
127 (74) |
The effect of postweaning diet is dependent on leptin treatment (or vice versa) |
|
ABC (3-way interaction) |
7 (3) |
The effects of all three factors are mutually dependent |
| Uncategorised | 128 (59) | Although significant changes were detected by one-way ANOVA, no individual factor or interaction term was subsequently significant in a three-factor ANOVA |
Bracketed figures for each category indicate the number of genes in the category that showed at least 1.25 fold change in transcript abundance across the data set as a whole. Additional file 2: Table S2 shows expression data for all genes falling into each category.