Figure 5.

L-plastin phosphorylation is important for migration and invasion of tumor cells in vitro. (A) migration in the presence or (B) absence of collagen I and (C) invasion into matrigel of MV3, MV3 control, MV3 LPL and MV3 S5A LPL cells. Migration and invasion were analyzed using 10% FCS as chemoattractant as described in materials and methods. Cells were incubated 3 hours for migration assays and 18 hours for invasion assays. Left part: representative confocal laser scan microscopy of migrated cells. Right part: statistics of at least three independent experiments were calculated using one-way ANOVA, values represent means ± SEM (* = p < 0.05; ns = not significant).