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. 2014 Jan 13;14:24. doi: 10.1186/1471-2229-14-24

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Copyright © 2014 Fredotović et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Genomic in situ hybridisation (GISH) in Allium × cornutum; a, b) and subsequent 5S rDNA mapping (c) to mitotic metaphase chromosomes of Allium × cornutum. (a, b) GISH with genomic DNA of A. pskemense (green), A. roylei (red) and A. cepa as blocking DNA (DAPI-blue); (a) Arrows and letters (C, R, and P) indicate the putative parental origins of the three genomes (A. cepa, A. roylei and A. pskemense, respectively); (b, c) The letters C, R, and P indicate chromosomes carrying 5S (green) and 35S (red) signals that belong to the three different parental genomes. C genome The 35S rDNA-carrying chromosome could not be identified; therefore it is not indicated. One nucleus (top left corner) visible in (b) was lost during reprobing and is not visible in (c).