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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Hypertension. 2013 Sep 23;62(6):1068–1073. doi: 10.1161/HYPERTENSIONAHA.113.01514

Figure 3.

Figure 3

Circulating TH17 increases in response to placental ischemia in RUPP rats. Administration of IL-17 RC into RUPP rats blunted this increase. Circulating peripheral blood leukocytes (PBLs) isolated from normal pregnant (NP, n=9), placental ischemic (RUPP, n=10), and IL-17RC infused (RUPP + IL-17RC, n=12) rats were collected and analyzed by flow cytometry. Top panel (A) shows the graphed results indicating that TH17 cells (CD4+ RORγ+CD25−) are increased in RUPP compared to NP rats and are decreased in RUPP rats infused with IL-17RC (*P<0.05). The percent of CD4+RORγ+CD25− staining cells above the negative control was collected for each individual rat respectively and mean values for each experimental group (NP, RUPP, and RUPP + IL17-RC) was calculated. Panels (B–D) illustrate representative scatter plots and staining profiles of PBL triple stained with anti-CD4, anti-CD25, and anti-RORγ.