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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Cell Rep. 2013 Dec 27;6(1):117–129. doi: 10.1016/j.celrep.2013.11.043

Figure 6. JAM-A correlates with glioma malignancy and is informative for patient survival.

Figure 6

Box and whisker plots of expression level for JAM-A using Oncomine (https://www.oncomine.org/) demonstrates significantly higher expression (p<0.001 as assessed by one-way ANOVA) of JAM-A in glioblastoma tissue than in neural progenitor cells (A) and significantly higher expression (p<0.001 as assessed by one-way ANOVA) of JAM-A in glioblastoma tissue as compared with oligodendroglia (Oligo) tumors, low grade astrocytoma, or mixed glioma (oligodendroglia (Oligo) and astrocytoma (Astro)) (B). JAM-A is also informative for GBM patient survival as demonstrated by Kaplan-Meier survival plots (C). The red line indicates the high expression group (10 patients) with greater than one standard deviation above the mean of all patients. The blue line indicates the low expression group (9 patients) with greater than one standard deviation below the mean of all patients. The black line indicates the group (57 patients) within one standard deviation of mean. Kaplan-Meier survival plots for glioma (D) and GBM (E) patients with differential tumor JAM-A expression using the NCI REMBRANDT database at a level of two-fold upregulation indicates that JAM-A levels inversely correlates with patient survival (red line indicates two-fold upregulation (137 glioma patients, 70 GBM patients), blue line indicates two-fold downregulation (8 glioma patients), and black line represents intermediate expression (198 glioma patients, 110 GBM patients). Analysis of JAM-A using histochemical staining in a patient dataset (Odense dataset, Odense University Hospital Denmark) indicates that JAM-A is elevated in GBM as compared to normal brain controls (F) and that high JAM-A expression correlates with poor patient prognosis (G; 102 patients in the JAM-A high group and 83 patients in the JAM-A low group). Data for all graphs displayed as mean values +/− standard deviation, ** p < 0.01 and *** p < 0.001 as assessed by one-way ANOVA. For survival analysis, the log-rank p-value for significance of difference between groups is indicated on each individual graph. See also Figure S5.