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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Immunol. 2013 Dec 30;192(3):1055–1063. doi: 10.4049/jimmunol.1302694

Figure 1.

Figure 1

A single locus on mouse chromosome 12 strongly influences the development of Vδ6.3+ γδ T cells in Id3-deficient mice. (A) Deficiency of Id3 induces the accumulation of a large population of Vδ6.3+ γδ T cells in the thymus of mice with pure B6 background. However, this phenomenon persists in some but is absent in other mice with a B6/129 mix background, as two representative mice are showing here. (B) A breeding scheme was established to dissect potential modifying gene(s) in the B6 and 129 genetic backgrounds. Only F2 B6/129 hybrid Id3−/− mice were used in the linkage analysis. (C) Percentages of Vδ6.3+ γδ T cells among total thymocytes were scored for individual mice of indicated genotype group. Each dot represents one mouse. Horizontal line indicates mean of the genotype group. (D) SNP analysis of the B6/129 hybrid Id3−/− mice showed that one location on chromosome 12 is strongly correlated with the presence of more than 1% of Vδ6.3+ γδ T cells in the thymus. (E) Detailed SNP analysis of four mice with more than 1% of Vδ6.3+ γδ T cells in the thymus showed a linkage to B6 homozygocity within a 3 Mb region on chromosome 12. (F) A map of known features around the critical region on chromosome 12; arrows indicate the border of the region (25.16Mb-28.02Mb) as determined in E. Note that Id2 and Kidins220 are immediately outside of the border of this region.