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. 2014 Jan 23;4:3838. doi: 10.1038/srep03838

Figure 3. Effects of SAM and sinefungin cofactor on SAM-depleted Tth111II endonuclease activity.

Figure 3

Panel (A), SAM-depleted Tth111II digestion of 1 μg pBR322. Panel (B), same as in (A), but with addition of 320 μM SAM. Panel (C), same as in (A), plus 1 mM sinefungin. (D), untreated Tth111II with internally bound SAM. Reaction was carried out in Tth Buffer, 65°C for 30 min. The triangle on top of lanes represents a 2-fold serial dilution of the enzyme (starting at 11.4 pmole). NC, nicked circular; L, linear, SC, supercoiled DNA.