Figure 3.

EIF5A2 KD inhibits melanoma cell invasion by reducing MMP-2 activity. MMRU melanoma cell lines were transfected with either control siRNA (siC) or siEIF5A2. (A) Protein extracts were prepared 72 h after transfection and analysed for the expression of EIF5A2. The numbers below the blot indicate fold change of EIF5A2 expression level. (B) For Boyden chamber assay, 48 h after siRNA transfection, cells were suspended in serum-free medium and seeded on matrigel, incubated at 37 °C for 24 h, stained with crystal violet and quantified. *P<0.05, Student's t-test. (C) Matrix metalloproteinase-2 activity determined by zymography, which was performed 48 h after transfection. Recombinant MMP-2 was used as a positive control. The numbers below the gel picture indicate fold change of MMP-2 activity.