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. 2013 Oct 31;110(2):399–408. doi: 10.1038/bjc.2013.688

Figure 5.

Figure 5

Integrin-linked kinase KD or PTEN overexpression decrease EIF5A2 expression and invasion in MMRU melanoma cells. (A) Control or shILK-KD MMRU melanoma cells were subjected to western blot analysis and analysed for the expression of EIF5A2, p-Akt, total Akt and ILK. (B) Boyden chamber assay was performed 24 h after transfection. *P<0.05, Student's t-test. (C) Quantitative RT–PCR analysis of EIF5A2 from total RNA extraction in control and shILK-KD MMRU cells. ***P<0.001, Student's t-test. (D) MMRU melanoma cells were transfected with either vector control or PTEN overexpression plasmid. Protein extracts were prepared 48 h after transfection and analysed for expression of EIF5A2, p-Akt, total Akt, ILK and PTEN by western blotting. (E) Boyden chamber assay was performed 24 h after transfection. **P<0.01, Student's t-test.