Table 1. Detailed information about tissue samples used in this study.
Sample | Tissue type/origin | Duration of cultivation | Slices acquired with | Fixation time | |
---|---|---|---|---|---|
**200111 |
Carcinoma of the larynx |
5 h, 6d |
Method establishment |
Vibratome |
None (tissue not cultivated) |
**190411 |
Carcinoma of the trachea |
1d, 2d, 6d, 9d, 12d |
Method establishment |
Vibratome |
24 h |
**270611 |
Carcinoma of the tonsil |
6d |
See data (Figures 1A and 1B) |
Vibratome |
24 h |
**080611 |
Metastasis of an oropharyngeal carcinoma |
5d, 7d |
See data (Figure 1B) |
Vibratome |
48 h |
**120911 |
Metastasis of a carcinoma of the tonsil |
6d |
See data (Figure 3) |
Tissue chopper |
18 h |
**071111 |
Carcinoma of the base of tongue |
3d, 6d |
See data (Figures 1C and 5) |
Tissue chopper |
24 h |
**070512 |
Carcinoma of the larynx |
4d |
Method/analysis establishment |
Tissue chopper |
24–48 h |
**160512 |
Carcinoma of the base of tongue |
4d |
Method/analysis establishment |
Tissue chopper |
24–48 h |
**240512 |
Carcinoma of the base of tongue |
4d |
Method/analysis establishment |
Tissue chopper |
24–48 h |
**020712 |
Metastasis of a supraglottic carcinoma |
3d, 5d |
See data (Figure 4) |
Tissue chopper |
24–48 h |
**040713 |
Carcinoma of the pharynx |
3d, 5d |
See data (Figures 1A and 1C) |
Tissue chopper |
24 h |
**040713 | Metastasis of a carcinoma of the pharynx | 3d, 5d | See data (Figures 1A, 2A and 2B) | Tissue chopper | 24 h |
This table presents the type of the tumour tissues used in this study, the duration of cultivation, the cutting method, the use of each tumour and where the data are shown as well as the fixation time.
The approach in our study was the following: We first tested vibratome sections (samples **200111, **190411, **270611 and **080611) of which only three could be kept in culture with good preservation until day 6 (**200111, **270611, **080611). As we found evident deterioration at day 7 in sample **080611, we did not focus any longer on establishing a maximal survival time for individual tumours. Our reasoning was that for the purpose of the method, it is much more important to establish a minimum survival time suitable to test drug effects. We found that slices cut with a tissue chopper were faster to obtain and more uniform in thickness and exhibited very good tissue preservation until day 6. Therefore, we looked at the effects of treatments on day 4 (samples **070512, **160512 and **240512) or – if there was enough tissue – on days 3 and 5 (samples **020712, **040713TM and **040713Met).