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. Author manuscript; available in PMC: 2014 Oct 29.
Published in final edited form as: Circulation. 2013 Sep 27;128(18):2047–2057. doi: 10.1161/CIRCULATIONAHA.113.002887

Figure 5.

Figure 5

TET2 modulation alters 5-hmC levels and the intimal hyperplastic response to arterial injury. (A) EVG staining 3 weeks post-injury of femoral arteries transduced with either a control, TET2 knockdown or TET2 overexpressing virus. (B) Quantitation of the neointima/media ratio in each group. (C) RNA was isolated from the neointima of the control, TET2 knockdown or TET2 overexpression injured mice using the Pinpoint Isolation System and gene expression for Tet2, Myocd and Myh11 in each group was determined by qPCR. (D) Representative images of Myh11, Tet2 and 5-hmC immunostaining from each group. Boxed areas are enlarged on the right. Scale bar, 50 μm. n represent the number of individual mice analyzed. Two or more slides (12–18 sections) per animal were used for analysis. Data are presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001 compared to the control group.