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. 2014 Feb 1;141(3):697–706. doi: 10.1242/dev.103978

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Re-feeding promotes reconstitution of the CMZ. (A,B) Explanted retinas from ND embryos grown in L15 medium containing sugars and amino acids for 24 hours showed an increase in CMZ proliferation after a 2-hour pulse with EdU. n=3, 18 retinas/condition. (C) This increase in proliferation varied with nutrient levels, suggesting that nutrients can regulate proliferation within a dynamic range. n=3, 18 retinas/condition. (D,E) Proliferation was initiated in the CMZ as early as 20 minutes after re-feeding (D), with robust proliferation being observed 2 hours after the addition of nutrients (E). (F,G) After culture for 24 hours in L15, a strong upregulation of Cyclin D1 and the proneural genes ascl1 and atoh7 was observed in re-fed retinas. n=3, 18 retinas/condition. (B,C,G) Error bars show 95% confidence intervals; ^***P≤0.001 (unpaired t-test). Scale bars: 50 μm (A,D,E,F).