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. 2014 Feb 1;141(3):697–706. doi: 10.1242/dev.103978

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Activation of mTOR by nutrients is necessary for neural commitment and differentiation but not for the maintenance of the most immature stem/progenitor cells. (A,B) Proneural gene expression levels were decreased in ND and rapamycin-treated embryos (A), as were levels of the progenitor markers sox2 and n-Myc (B). n=8, 32 embryos/condition. (C) Expression of the eye field transcription factor gene rx-1 remained unchanged by ND. n=4, 16 embryos/condition. (D) c-Myc expression levels remained unchanged; however, the domain of c-Myc expression was reduced in the central CMZ of ND and rapamycin-treated embryos. n=8, 32 embryos/condition). (E) Expression levels of the stem cell marker genes hes1 and hes4 remained unchanged (n=4, 16 embryos/condition. In all cases, error bars show 95% confidence intervals; ^***P≤0.001, ^**P≤0.01, ^*P≤0.05 (unpaired t-test).