Fig. 8.

Activation of mTOR signalling is sufficient to initiate, but not maintain, progenitor proliferation. (A-C) Eye size at stage 41 was not rescued by bpV (A), nor was proliferation (B), despite expression of Cyclin D1 being significantly higher than in ND embryos (C). n=4, 16 embryos/condition. Error bars show 95% confidence intervals; ^*P≤0.05 (unpaired t-test). (D,E) In explanted cultures a short-term incubation (2 hours) with bpV was sufficient to initiate proliferation in ND retinas; however, bpV treatment was not sufficient to maintain proliferation for longer periods. n=4, 20 retinas/condition. Scale bars: 50 μm (E,F).