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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: J Am Chem Soc. 2013 Nov 6;135(44):16632–16640. doi: 10.1021/ja4085918

Figure 3.

Figure 3

Concentration dependence of SssI methyltransferase activity. Chips were modified in all quadrants with the unmethylated BstUI 20-mer. DNA protection by various concentrations of SssI was evaluated side by side on the same chip, and CV scans from representative electrodes are shown overlaid (inset). Overlaid CV traces include the initial signal before BstUI treatment (blue), 8 nM SssI (red), 4 nM SssI (orange), 2 nM SssI (green), and untreated (purple). Quantification of the observed signal protection is displayed as an SssI activity curve where the circled points correspond to the concentrations represented by the overlaid CV traces. Error bars represent the standard deviation across 4–8 electrodes tested for each SssI concentration on 4 chips. All CV scans were performed in M/R buffer (10 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, pH 7.9) with an Ag/AgCl reference electrode at a 100 mV/s scan rate.