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Lumen formation by each mechanism in different vessel types. (a–i) Lumen formation by cell–cell repulsion, vacuolation or the combined mechanism from left to right for an initial vessel thickness of one cell (top row), two cells (second row) or three cells (third row) at MCS 10 000. (j,k). Time-lapse images (MCS 500, 1000 and 2000) of lumen formation by the combined mechanism in a one-cell-thick vessel and in a two-cell-thick vessel.
