Figure 3.

Experimental designs. For some in vitro studies, human skeletal muscle derived cells were seeded in parenchymal spaces while microvessel perfusate was supplemented with a myotoxic drug (Doxorubicin); in other in vitro studies, microvessels were seeded with human umbilical vein endothelial cells. For in vivo studies, full-thickness punches of the scaffolds with or without both cell types were implanted subcutaneously or on the mesentery of the small intestine in nude rats.