Skeletal muscle cell differentiation on microvessel scaffolds. Confocal
images of hSkMDCs (A–D) in the parenchymal space and (E–H) on
glass coverslips; immunostaining for (A,E) F-actin (green), (B,F) desmin (red),
(C,G) F-actin merged with desmin, with nuclear counterstain (blue), and (D,H)
epifluorescence images after immunostaining for sarcomeric α-actin.
Arrows point to a multi-nucleated cell. Scale bars: (A–C, E–G)
10 µm; (D, H) 5 µm.