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Aβ(1–42) but not Aβ(1–40) fibrils enhance HIV-1 infection of TZM-bl cells. Equal amounts of the dual-tropic HIV-1 lab strain NL4-3 PI 952 [11] were pre-incubated for 5 min at RT with the indicated concentrations of Aβ(1–42) or Aβ(1–40) fibrils, which were incubated for four and six days, respectively of oligomerization. Subsequently, the pretreated viruses were used to infect TZM-bl reporter cells and infection-induced luciferase activity was assayed 48 h post infection.
