Table 1.
The sources of the antibodies used, and respective pre-treatment and dilution
| Antibody | Dilution | Pre-treatment | Supplier | Code |
|---|---|---|---|---|
| Ankyrin-G |
1:100 |
H2(20) |
NeuroMab |
75-146 |
| APP |
1:7500 |
H2(10) |
Chemicon |
MAB348 |
| Calbindin |
1:250 |
H2(20) |
Sigma |
C7354 |
| Calretinin |
1:100 |
H1(20) |
Novocastra |
NCL-CALRETININ |
| CD3 |
1:50 |
H2(30) |
Dako |
M7254 |
| CD34 |
1:150 |
H2(20) |
Dako |
M7165 |
| CD45 (LCA) |
1:200 |
H1(30) |
Dako |
M0701 |
| CD68 (PGM1) |
1:50 |
H2(20) |
Dako |
M0876 |
| Chromogranin |
1:100 |
H2(20) |
Novocastra |
NCL-CHROM-430 |
| GFAP |
1:1000 |
H1(5) |
Dako |
Z0334 |
| HLA-DR (cr3/43) |
1:300 |
H2(20) |
Dako |
M0775 |
| HuC/HuD |
1:160 |
H1 (30) |
Molecular probes |
A-21271 |
| IDH1 |
1:50 |
H1(20) |
Dianova |
DIA H09 L |
| Ki67 |
1:100 |
H2(20) |
Dako |
M7240 |
| Nestin |
1:200 |
H2(20) |
Santa Cruz biotech |
sc-23927 |
| Neu N |
1:2000 |
H1(20) |
Chemicon |
MAB377 |
| NF (pan-NF) |
1:100 |
H2(30) |
Zymed |
18-0171Z |
| NF200 KD |
1:500 |
H1(5) |
Dako |
M0762 |
| p62 |
1:100 |
H2(20) |
BD Transduction labs |
610833 |
| Parvalbumin |
1:500 |
H2(20) |
Millipore (Chemicon) |
MAB1572 |
| Synaptophysin |
1:40 |
H2(20) |
Dako |
M0776 |
| Synuclein |
1:500 |
Offline* |
BD Transduction labs |
610787 |
| Tau (AT8) |
1:500 |
H1(20) |
Innogenetics |
MN1020 |
| TDP43-P | 1:1500 | H1(30) | Cosmo Bio Ltd. | TIP-PTD-P02 |
Pre-treatment codes: H1(5) = Epitope Retrieval Solution 1 (ER1) for 5 minutes; H1(10) = Epitope Retrieval Solution 1 (ER1) for 10 minutes; H2(20) = Epitope Retrieval Solution 2 (ER2) for 20 minutes; H2(30) = Epitope Retrieval Solution 1 (ER1) for 10 minutes. *Synuclein offline pre-treatment: 60 minutes in 80% Aqueous Formic Acid.