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. 2013 Dec 30;11:122. doi: 10.1186/1741-7007-11-122

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Copyright © 2013 Baumgart et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Two-dimensional thin-layer chromatography (2D-TLC) analysis of [¹⁴C]-labeled polar lipids from Corynebacterium glutamicum cells grown in glucose (A-F) or myo-inositol (G-H). (A) and (G)C. glutamicum ATCC 13032, (B) and (H) ATCC 13032 ΔipsA,(C) ATCC 13032 ΔipsA pAN6-cg3323,(D) ATCC 13032 ΔipsA::pK18int-ipsA,(E) ATCC 13032 ΔipsA pAN6-DIP1969 and (F) ATCC 13032 ΔipsA pAN6-Rv3575. The cells were cultured in CGXII with either glucose (A-F) or myo-inositol (G, H). PI, phosphatidylinositol; TMCM, trehalose monocorynomycolate; GMCM, glucose monocorynomycolate; Ac₁PIM₂, monoacylated phosphatidyl myo-inositol dimannoside; GlcAGroAc₂, 1,2-di-O-C₁₆/C_18:1-(α-d-glucopyranosyluronic acid)-(1→3)-glycerol (GL-A); ManGlcAGroAc₂ 1,2-di-O-C₁₆/C_18:1-(α-d-mannopyranosyl)-(1→4)-(α-d-glucopyranosyluronic acid)-(1→3)-glycerol (GL-X).