Table 1.
Transcriptome analysis of an IpsA deletion mutant
| Locus tag | Gene | Annotated function | Ratio |
|---|---|---|---|
| cg3323 |
ino1 |
Myo-inositol phosphate synthase |
0.047 |
| cg0044 |
rbsB |
ABC transporter/periplasmic d-ribose-binding protein |
0.082 |
| cg0045 |
- |
Probable ABC transport protein, membrane component |
0.138 |
| cg2910 |
- |
Transcriptional regulator, LacI family |
0.173 |
| cg3195 |
- |
Flavin-containing monooxygenase |
0.178 |
| cg0046 |
- |
Probable ABC transport protein, ATP-binding component |
0.323 |
| cg3210 |
- |
Cell envelope-related transcriptional regulator |
0.411 |
| cg1421 |
- |
Putative dinucleotide-binding enzyme |
5.214 |
| cg0533 |
menE |
O-succinylbenzoic acid-CoA ligase |
5.467 |
| cg0534 | - | Putative integral membrane protein | 5.597 |
mRNA ratios (ΔipsA/wild-type) of IpsA target genes in microarray experiments are shown. Presented are all genes where binding of IpsA could be shown in electrophoretic mobility shift assays (EMSAs) as well as IpsA itself, confirming the deletion.