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. 2013 Mar 20;4(1):86–100. doi: 10.3390/genes4010086

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Adenosine triphosphate (ATP) disrupts the interaction between Rtg2 and Mks1 in total cellular lysates. Cellular lysates prepared from yeast cells expressing Rtg2-myc and/or Mks1-HA as indicated were analyzed for the effect of ATP, ADP and adenosine 5′-(β,γ-imido) triphosphate (AMP-PNP) on the interaction between Rtg2 and Mks1 by immunoprecipitation, as described in the Experimental Section. Cellular lysates were treated with the nucleotides at indicated concentrations, and Rtg2-myc was immunoprecipitated using anti-myc anti-body and protein G Sepharose beads. Rtg2-myc and Mks1-HA in IP pellet fractions were detected by immunoblotting.