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. 2013 Mar 20;4(1):86–100. doi: 10.3390/genes4010086

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The effect of ATP titration on Mks1 release from Rtg2. (A) Immunopurified Rtg2-myc-Mks1-HA complex from RBY915 cells co-expressing Rtg2-myc and Mks1-HA was incubated with titrating levels of ATP, and the presence of Rtg2-myc and Mks1-HA in the pellet and supernatant (released) fractions were determined by Western-blotting. (B) Quantitative analysis of the amount of Mks1 released from Rtg2 in the presence of ATP. The result was the average of two independent experiments. (C) The effect of 5 mM ADP, GTP or GDP and 10 mM AMP-PNP or ATPγS on the interaction between Rtg2 and Mks1.