Table 2.
Plasmids used in this study.
| Plasmid | Description | Source | Application |
|---|---|---|---|
| pZL1480 | pRS416-MKS1p-MKS1-HA, expressing HA-tagged Mks1 from the MKS1 promoter. | This study | Figure 1, Figure 2, Figure 4 |
| pTS215 | pRS416-MKS1, expressing MKS1 from its own promoter. | [28] | Figure 1 |
| pFZ142 | pRS416-RTG2p-RTG2(Kla)-HA, expressing HA-tagged Rtg2 homolog from K. lactis from the RTG2 promoter of S. cerevisiae. | This study | Figure 3 |
| pFZ136 | pRS416-RTG2p-RTG2(Kwa)-HA, expressing HA-tagged Rtg2 homolog from K. waltii from the RTG2 promoter of S. cerevisiae. | This study | Figure 3 |
| pZL927 | pS416-MKS1, expressing Mks1 from the endogenous promoter. | [28] | Figure 4 |
| pFZ138 | pS416-MKS1p-MKS1(Kla), expressing the Mks1 homolog from K. lactis from the MKS1 promoter of S. cerevisiae. | This study | Figure 4 |
| pFZ144 | pRS416-MKS1p-MKS1(Kwa), expressing the Mks1 homolog from K. waltii from the MKS1 promoter of S. cerevisiae. | This study | Figure 4 |
| pFZ134 | pRS416-MKS1p-MKS1(Kla)-HA3, expressing HA-tagged Mks1 homolog from K. lactis from the MKS1 promoter of S. cerevisiae. | This study | Figure 4 |
| pFL150 | pRS416-MKS1p-MKS1(Kwa)-HA3, expressing HA-tagged Mks1 homolog from K. waltii from the MKS1 promoter of S. cerevisiae. | This study | Figure 4 |
| pZL1951 | pRS415-RTG2-myc, expressing myc-tagged Rtg2 from the RTG2 promoter. | This study | Figure 5 |
| pFZ140 | pRS415-RTG2p-RTG2(Kla)-myc, expressing myc-tagged Rtg2 homolog from K. lactis from the RTG2 promoter of S. cerevisiae. | This study | Figure 5 |
| pFZ148 | pRS415-RTG2p-RTG2(Kwa)-myc, expressing myc-tagged Rtg2 homolog from K. waltii from the RTG2 promoter of S. cerevisiae. | This study | Figure 5 |