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. 2013 Jun 7;4(2):293–305. doi: 10.3390/genes4020293

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Double immunofluorescence of SOX9 (red) and cytokeratin (green) in gonads from L. olivacea in ovo. (A) Gonads of embryos incubated at female-promoting temperature (FPT) Miller’s stage (st) 29 and (B) male-promoting temperature (MPT) st 23.