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Double immunofluorescence of SOX9 (red) and cytokeratin (green) in gonads from L. olivacea cultured with (A) Opti-MEM, CO, (B) Lipofectamin and Opti-MEM, CL, (C) transfected with the scrambled control sequence, CS, and (D–F) transfected with 100, 300 and 600 pmol of siRNA, respectively. Scale bar = 20 µm.
