Figure 4.
Expression of Ca2+ channels and transporters in control and DM1 myotubes at 10 (T10) and 15 (T15) days of differentiation. (A) Representative images of SERCA2 (green) and RyR1 (red), nuclei (blue-Dapi) immunofluorescence of T15 differentiated control and DM1 myotubes. Scale bar is 10 μm. (B) Representative Western blot analysis of RyR1, DHPR α1s (Cav1.1) and SERCA2 of control and DM1 myotubes at 10 and 15 days of differentiation. (C–E) Quantification of RyR1, DHPRα1s and SERCA2 protein levels normalized to β-actin. Data are the mean ± SD of two experiments. ** p < 0.01 as compared to controls by Students’ t test. The numbers of single lines studied is given in brackets.