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. 2012 Oct 12;3(4):615–633. doi: 10.3390/genes3040615

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Potential combinations of Sleeping Beauty components. (A) Cytoplasmic injection of an expression plasmid encoding Sleeping Beauty transposase and a plasmid carrying a SB-transposon; (B) Cytoplasmic injection of Sleeping Beauty-mRNA and a SB-transposon; (C) Injection of recombinant Sleeping Beauty protein and a SB-transposon; (D) In all possibilities stable transposition into a chromosome occurs, however, it is likely that A-C differ in the kinetics and potential remobilization events. CMV-SB100x, cytomegalovirus immediate early promoter driving a SB100x-cDNA; CAGGS, cytomegalovirus enhancer, chicken beta actin hybrid promoter driving a Venus-cDNA; ITR, SB specific-inverted terminal repeat; blue triangles, heterospecific loxP sites for a potential recombinase-mediated cassette exchange [25].