Figure 2.

Transposon transgenic pig expressing Venus reporter. (A) Boar #505 under specific Venus excitation at the age of four weeks, Venus fluorescence is indicated by green color. Note the blue appearance of the hands holding the pig is due to reflected and scattered excitation light; (B) Scheme of CPI injection. After injection of the plasmid solution into the cytoplasm (I.), the plasmids translocate into the nucleus (II.). Expression of the SB100× helper plasmid results in the production of active SB protein and specific transposition of the transposon (without plasmid backbone) into the genome (III.).