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Expression of Venus fluorescence in spermatozoa. (A) Specific Venus fluorescence of a spermatozoon from F0 boar #503; (B) Brightfield image of A; (C) Flow cytometric analysis of pig spermatozoa. The black line in the FACS analysis represents wild type sperm; blue line, spermatozoa from F1 boar with single integration; red line, spermatozoa from boar #503 with three integrations. Note that all spermatozoa from the transgenic pigs are uniformly Venus-positive.
