Figure 4.

The EWS/FLI chimera possesses unique DNA binding affinities and biological properties distinct from native ETS family members. Both high- and low-affinity ETS DNA binding sites are characterized by a core ACCGGAA/T consensus sequence facilitating both ETS-redundant and ETS-divergent transcriptional regulation. In Ewing sarcoma, EWS/FLI also binds the traditional ETS-consensus sequence, but shows increased preferencefor a GGAA-containing microsatellite. In certain upregulated targets, this GGAA microsatellite response element is required for DNA binding and gene activation, which proportionately increases with an increasing number of GGAA motifs. “TF” = transcription factor.