Figure 1.

BSK3 Interacts with Multiple Receptor-like Kinases.

(A) Confirmation of interactions between BSK3 and LRR–RLKs using co-immunoprecipitation. The BSK3–Myc–His fusion protein was co-expressed with the indicated BSK3-interacting proteins tagged by GFP, using transient transformation of tobacco leaves. The GFP fusion proteins were immunoprecipitated using an anti-GFP antibody, and the immunoblots was probed with anti-Myc antibody first and then anti-GFP antibody.

(B) Confirmation of direct interactions between BSK3 and BSR850 using BiFC.

(C) Confirmation of direct interactions between BSK3 and BSR850 using the overlay assay. Gel blot of indicated proteins was probed with GST–BSK3 and anti-GST antibody.

(D) BR increases the expression levels of several BSK3-interacting LRR–RLKs. Total RNAs were extracted from 7-day-old Col-0 seedlings grown on half-strength MS media containing indicating concentrations of eBL. Expression levels of the indicated genes were analyzed using quantitative RT–PCR and normalized to the UBQ5 gene. Error bars are standard errors of three biological replicates.