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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Mol Cell. 2013 Dec 5;52(6):832–843. doi: 10.1016/j.molcel.2013.11.005

Figure 5.

Figure 5

FRQ stability is independently determined by interactions with FRH and FWD-1. (A) Strain genotypes: Linkage group 7; at the frq locus: V5H6 tagged frq with the FFD (FRQ-FRH Interaction domain) intact or deleted; at the fwd-1 locus: fwd-1 or hph. (B) Total levels of FRQ during its degradation were followed in epitope tagged strains with or without the FRQ FFD (frq and frq Δ FFD) and/or with or without FWD-1 (frq, Δ fwd-1 and frq Δ FFD, Δ fwd-1) after addition of CHX (to inhibit translation). 10μg of total protein was loaded per lane and membranes (mem) stained with Amido Black were used as loading control. (C) Densitometric analysis of data shown in (B) was used to calculate half-life. For each strain, time zero was set to 100%, allowing degradation rate to be analyzed relative to the other strains. Half-life was calculated by least squares regression of the line through the data points with the resulting equation and goodness of fit (R2) shown. Data are means ± S.E., n = 3.